VARIATIONS IN NORMAL AND BRUCELLA-IMMUNE RABBIT SERUM AS DETERMINED BY PAPER-PARTITION CHROMATOGRAPHY

Corticosteroids in urine of normal persons determined by paper chromatography.

The presence in human urine of small amounts of material with adrenocortical activity is well established (1, 2). The chemical characterization of this urinary material by the classical methods used for the steroids in adrenal glands has been technically difficult. In the six reported instances (3-8) in which corticoids have been isolated and identified by these procedures, extracts of large vo...

The phosphatides of human plasma. I. Normal values determined by paper and column chromatography.

The Fractionation of Rabbit y-Globulin by Partition Chromatography

Friedkin, M. & Lehninger, A. L. (1949). J. biol. Chem. 178, 611. Holton, F. A. (1954a). Biochem. J. 58, i. Holton, F. A. (1954b). Dissertation for Ph.D., Cambridge University. Horecker, B. L. & Kornberg, A. (1946). J. biol. Chem. 165, 11. Hunter, F. E. (1951). Phophorus Metabolism, 1, 297. Eds. W. D. McElroy and Bentley Glass. Baltimore: The Johns Hopkins Press. Judah, J. D. (1951). Biochem. J....

The quantitative separation of estrogens by paper partition chromatography.

The varying success of known chromat’ographic procedures for the separation and identification of microquantities of estrogens (1, 2) has led to the development of paper partit!ion chromatographic techniques which are reported here. These t’echniques permit rapid separation, identification, and quantitative estimation of free est.rogens in biological materials. The solvent systems of o-dichloro...

immune reactivity of brucella melitensis-vaccinated rabbit serum with recombinant omp31 and dnak proteins.

background and objectives: brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. materials and methods: in this study, in order to screen immunogenic candidate antigens for the development of a brucella subunit vaccine, a cytoplasmic protein (dnak) and an outer membrane protein (omp31) of b. melitensis were cloned, expres...